联系方式：

Email：lai_liangxue@gibh.ac.cn

教育背景

1980年9月---1984年7月  四川畜牧兽医学院兽医系 西兽医专业 农学学士

1984年9月---1987年7 月 中在国人民解放军兽医大学动物解剖学 农学硕士

1992年9 月---1995 年7 月 东北农业大学生物工程系 理学博士

工作经历

2007-现在，研究员，中国科学院广州生物医药与健康研究院

200６ 年12 月---2007 Senior ｅｍｂｒｙｏｌｏｇｉｓｔ　美国先进生殖治疗中心　

２００４年－２００６年　研究助理教授　美国密苏里大学国家猪资源保护与研究中心

２００２　年－２００4年　研究助理教授　美国密苏里大学动物研究中心

１９９８　年－２００２年　博士后　美国密苏里大学动物研究中心

１９９７　年－１９９８年　副教授　　解放军农牧大学

１９９５年－１９９７年　博士后　解放军农牧大学军事兽医研究所转基因动物实验室

１９９０年－１９９５年　讲师　中国人民解放军兽医大学动物解剖教研室

１９８７年－１９９０年　　助教　中国人民解放军兽医大学动物解剖教研室

研究经历

1. 在2001-2008年期间，将分子技术与动物克隆技术相结合，成功地建立了12种转基因克隆猪。其中包括半乳糖苷转移酶单基因敲除克隆猪，半乳糖苷转移酶双基因敲除克隆猪，肝脏表达胸腺激酶克隆猪，全身表达Decay 基因克隆猪，全身表达绿色荧光蛋白克隆猪，内皮细胞过度表达eNOSE 基因克隆猪，eNOSE 基因敲除克隆猪，乳腺表达凝血因子VIII克隆猪，乳腺表达凝血因子IX克隆猪， 全身表达 Omega-3 脂肪酸（鱼油）克隆猪，，同时表达四种荧光的克隆猪，含有抗猪瘟病毒基因的克隆猪。

2. 华南地区特种疾病胚胎干细胞库的建立（2006-2011）

3. 基因敲除肌抑制素基因的克隆猪建立（2004-2007）。

4. 以孤雌胎儿细胞作供体核构建的核移植重组胚的发育潜能 （2003-2005）

5. 供体细胞线粒体对克隆胚胎发育的影响（2005-2007）。

6. 体细胞克隆的影响因素的探讨（1998-present.）。

7. 单精子注射猪的研究（2000.）

8. 表达人组织性纤溶酶原激活剂的转基因小鼠和家兔的建立 （1995-1997）。

9. 通过体内转染精原干细胞建立转基因小鼠. （1996-1998）。

10. 小鼠胚胎肝细胞神经元的脑内移植（ 1995-1998.）。

11. 精原干细胞的体外培养（.1996-1997.）。

12. 家兔胚胎干细胞的建立（1992-1995.）。

13. 鸡脑室室管膜的发育（. 1990-1992）。

14. 体外培养脑纹状体神经元的生物学特征1988-1990.。

15. 鸡中脑中央灰质的光电镜结构的研究（. 1984-1987）。

 荣誉和奖励

1.美国《科技新时代》杂志“医学技术最高成就奖”得者 2002 年（Popular Science’s Medical Tech Grand Award Winner. The best of what’s new- Knockout pigs- The most significant advance i n medical technology for the year 2002.）

2.美国《发现》杂志评出的2002年世界100大科技新闻中我们的基因敲除克隆猪排名第36。

3.美国发现杂志评出的2006年世界100大科技新闻中我们的转基因鱼油猪克隆猪排名第38

38）

4.我们的转基因绿猪照片和文字介绍被刊登在美国《国家地理杂志》 2005 年（National Geographic Magazine, Photo and story on transgenic pigs expressing the enhanced green fluorescent protein. July 2005, page 18）.

5.中国海外杰出青年基金获得者，2004-2007

论文发表情况

1. Xiao Y, Wei X, Jiang Z, Wang X, Ye W, Liu X, Zhang M, Xu Y, Wu D, Lai L, Yao H, Liu Z, Cao S, Liu P, Xu B, Li Y, Yao Y, Pei D, Li P. Loss of Angiopoietin-like 7 diminishes the regeneration capacity of hematopoietic stem and progenitor cells.J Hematol Oncol. 2015 Feb 6;8(1):7. [Epub ahead of print]

2. Xiao Y, Jiang Z, Li Y, Ye W, Jia B, Zhang M, Xu Y, Wu D, Lai L, Chen Y, Chang Y, Huang X, Liu H, Qing G, Liu P, Li Y, Xu B, Zhong M, Yao Y, Pei D, Li P. ANGPTL7 regulates the expansion and repopulation of human hematopoietic stem and progenitor cells.Haematologica. 2015 Jan 30. pii: haematol.2014.118612. [Epub ahead of print]

3. Yuan L, Wang A, Yao C, Huang Y, Duan F, Lv Q, Wang D, Ouyang H, Li Z, Lai L1..Aberrant expression of xist in aborted porcine fetuses derived from somatic cell nuclear transfer embryos. Int J Mol Sci. 2014 Nov 25;15(12):21631-43. doi: 10.3390/ijms151221631

4. Zhou X, Xin J, Fan N, Zou Q, Huang J, Ouyang Z, Zhao Y, Zhao B, Liu Z, Lai S, Yi X, Guo L, Esteban MA, Zeng Y, Yang H, Lai L Generation of CRISPR/Cas9-mediated gene-targeted pigs via somatic cell nuclear transfer. Cell Mol Life Sci. 2014 Oct 2. [Epub ahead of print].

5. Quan L, Chen Y, Song J, Yan Q, Zhang Q, Lai S, Fan N, Xin J, Zou Q, Lai L.Establishment of a Rabbit Oct4 Promoter-Based EGFP Reporter System.PLoS One. 2014 Oct 31;9(10).

6. Yan Q, Zhang Q, Yang H, Zou Q, Tang C, Fan N and Lai L. Generation of multi-gene knockout rabbits using the Cas9/gRNA system. Cell Regeneration 2014, 3:12 (27 September 2014).

7. Yan Q, Yang H, Yang D, Zhao B, Ouyang Z, Liu Z, Fan N, Ouyang H et al . Production of transgenic pigs over-expressing the antiviral gene Mx1. Cell Regeneration 2014, 3:11 (26 September 2014).

8. Wang Y, Yang HQ, Jiang W, Fan NN, Zhao BT, Ou-Yang Z, Liu ZM, Zhao Y, Yang DS, Zhou XY, Shang HT, Wang LL, Xiang PY, Ge LP, Wei H, Lai LX. Transgenic expression of human cytoxic T-lymphocyte associated antigen4-Immunoglobulin (hCTLA4Ig) by porcine skin for xenogeneic skin grafting. Transgenic Res. 2014 Sep 19. [Epub ahead of print]

9. Wang H, Song C, Gurel Z, Song N, Ma J, Ouyang H, Lai L, Payne KJ, Dovat S. Protein Phosphatase 1 (PP1) and Casein Kinase II (CK2) regulate Ikaros-mediated repression of TdT in thymocytes and T-cell leukemia. Pediatr Blood Cancer. 2014 Sep 11. doi: 1002/pbc.25221. [Epub ahead of print]

10. Huang J, Guo X, Fan N, Song J, Zhao B, Ouyang Z, Liu Z, Zhao Y, Yan Q, Yi X, Schambach A, Frampton J, Esteban MA, Yang D, Yang H, Lai L.RAG1/2 Knockout Pigs with Severe Combined Immunodeficiency.J Immunol. 2014 Jun 27. pii: 1400915. [Epub ahead of print]

11. Wang D, Chen X, Song Y, Lv Q, Lai L, Li Z. Disruption of imprinted gene expression and DNA methylation status in porcine parthenogenetic fetuses and placentas.Gene. 2014 Sep 1;547(2):351-8. doi: 10.1016/j.gene.2014.06.059. Epub 2014 Jun 27.

12. Wang D, Song Y, Huang Y, Duan F, Lv Q, Ouyang H, Lai L, Li Z. Genomic imprinting analysis of Igf2/H19 in porcine cloned fetuses using parthenogenetic somatic cells as nuclear donors.Biotechnol Lett. 2014 Jun 15. [Epub ahead of print]

13. Sun H, Hou Z, Yang H, Meng M, Li P, Zou Q, Yang L, Chen Y, Chai H, Zhong H, Yang ZZ, Zhao J, Lai L, Jiang X, Xiao Z. Multiple systemic transplantations of human amniotic mesenchymal stem cells exert therapeutic effects in an ALS mouse model.Cell Tissue Res. 2014 Jun 8. [Epub ahead of print]

14. Wang H, Ouyang H, Lai L, Petrovic-Dovat L, Stankov K, Bogdanovic G, Dovat S. Pathogenesis and regulation of cellular proliferation in acute lymphoblastic leukemia - the role of Ikaros.J BUON. 2014 Jan-Mar;19(1):22-8. Review.

15. Yang H, Wang G, Sun H, Shu R, Liu T, Wang CE, Liu Z, Zhao Y, Zhao B, Ouyang Z, Yang D, Huang J, Zhou Y, Li S, Jiang X, Xiao Z, Li XJ, Lai L. Species-dependent neuropathology in transgenic SOD1 pigs.Cell Res. 2014 Apr;24(4):464-81. doi: 10.1038/cr.2014.25. Epub 2014 Feb 28.

16. Li X, Yang Y, Bu L, Guo X, Tang C, Song J, Fan N, Zhao B, Ouyang Z, Liu Z, Zhao Y, Yi X, Quan L, Liu S, Yang Z, Ouyang H, Chen YE, Wang Z, Lai L. Rosa26-targeted swine models for stable gene over-expression and Cre-mediated lineage tracing.Cell Res. 2014 Apr;24(4):501-4. doi: 10.1038/cr.2014.15. Epub 2014 Feb 7. No abstract available.

17. Yang D, Xu J, Zhu T, Fan J, Lai L, Zhang J, Chen YE. Effective gene targeting in rabbits using RNA-guided Cas9 nucleases.J Mol Cell Biol. 2014 Feb;6(1):97-9. doi: 10.1093/jmcb/mjt047. Epub 2014 Jan 8.

18. Zou Q, Yan Q, Zhong J, Wang K, Sun H, Yi X, Lai L. Direct conversion of human fibroblasts into neuronal restricted progenitors.J Biol Chem. 2014 Feb 21;289(8):5250-60. doi: 10.1074/jbc.M113.516112. Epub 2014 Jan 2.

19. Yan S, Wang CE, Wei W, Gaertig MA, Lai L, Li S, Li XJ. TDP-43 causes differential pathology in neuronal versus glial cells in the mouse brain.Hum Mol Genet. 2014 May 15;23(10):2678-93. doi: 10.1093/hmg/ddt662. Epub 2013 Dec 30.

20. Xin J, Yang H, Fan N, Zhao B, Ouyang Z, Liu Z, Zhao Y, Li X, Song J, Yang Y, Zou Q, Yan Q, Zeng Y, Lai LHighly efficient generation of GGTA1 biallelic knockout inbred mini-pigs with TALENs.PLoS One. 2013 Dec 17;8(12):e84250. doi: 10.1371/journal.pone.0084250. eCollection 2013.

21. Huang Y, Ouyang H, Yu H, Lai L, Pang D, Li Z. Efficiency of porcine somatic cell nuclear transfer - a retrospective study of factors related to embryo recipient and embryos transferred.Biol Open. 2013 Oct 2;2(11):1223-8. doi: 10.1242/bio.20135983. eCollection 2013.

22. Zhang L, Tang J, Haines CJ, Feng H, Lai L, Teng X, Han Y. c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation.BMCDev Biol. 2013 Oct 27;13:38. doi: 10.1186/1471-213X-13-38.

23. Yan S, Song H, Pang D, Zou Q, Li L, Yan Q, Fan N, Zhao X, Yu H, Li Z, Wang H, Gao F, Ouyang H, Lai L. Expression of plant sweet protein brazzein in the milk of transgenic mice.PLoS One. 2013 Oct 14;8(10):e76769. doi: 10.1371/journal.pone.0076769.

24. Ji G, Ruan W, Liu K, Wang F, Sakellariou D, Chen J, Yang Y, Okuka M, Han J, Liu Z, Lai L, Gagos S, Xiao L, Deng H, Li N, Liu L. Telomere Reprogramming and Maintenance in Porcine iPS Cells.PLoS One. 2013 Sep 30;8(9):e74202. doi: 10.1371/journal.pone.0074202.

25. Fan N, Chen J, Shang Z, Dou H, Ji G, Zou Q, Wu L, He L, Wang F, Liu K, Liu N, Han J, Zhou Q, Pan D, Yang D, Zhao B, Ouyang Z, Liu Z, Zhao Y, Lin L, Zhong C, Wang Q, Wang S, Xu Y, Luan J, Liang Y, Yang Z, Li J, Lu C, Vajta G, Li Z, Ouyang H, Wang H, Wang Y, Yang Y, Liu Z, Wei H, Luan Z, Esteban MA, Deng H, Yang H, Pei D, Li N, Pei G, Liu L, Du Y, Xiao L, Lai L. Piglets cloned from induced pluripotent stem cells. Cell Res. 2013 Jan;23(1):162-6. doi: 10.1038/cr.2012.176. Epub 2012 Dec 18.

26. Han X, Ouyang H, Chen X, Huang Y, Song Y, Zhang M, Pang D, Lai L, Li Z. Aberrant expression of Igf2/H19 in porcine parthenogenetic fetuses and placentas.Anim Reprod Sci. 2013 Jun;139(1-4):101-8. doi: 10.1016/j.anireprosci.2013.04.008. Epub 2013 Apr 19.

27. Fan N, Lai L. Genetically modified pig models for human diseases.J Genet Genomics. 2013 Feb 20;40(2):67-73. doi: 10.1016/j.jgg.2012.07.014. Epub 2013 Jan 8.

28. Song J, Zhong J, Guo X, Chen Y, Zou Q, Huang J, Li X, Zhang Q, Jiang Z, Tang C, Yang H, Liu T, Li P, Pei D, Lai L.Generation of RAG 1- and 2-deficient rabbits by embryo microinjection of TALENs.Cell Res. 2013 Aug;23(8):1059-62.

29. Zhu J, Pang D, Zhou Y, Tang X, Huang Y, Xie W, Gao F, Lai L, Zhang M, Ouyang H. Direct conversion of porcine embryonic fibroblasts into adipocytes by chemical molecules.Cell Reprogram. 2012 Apr;14(2):99-105. doi: 10.1089/cell.2011.0074. Epub 2012 Feb 28.

30. Zhang L, Tang J, Haines CJ, Feng HL, Lai L, Teng X, Han Y. c-kit and its related genes in spermatogonial differentiation.Spermatogenesis. 2011 Jul;1(3):186-194. Epub 2011 Jul 1.

31. Fang X, Mou Y, Huang Z, Li Y, Han L, Zhang Y, Feng Y, Chen Y, Jiang X, Zhao W, Sun X, Xiong Z, Yang L, Liu H, Fan D, Mao L, Ren L, Liu C, Wang J, Li K, Wang G, Yang S, Lai L, Zhang G, Li Y, Wang J, Bolund L, Yang H, Wang J, Feng S, Li S, Du Y. The sequence and analysis of a Chinese pig genome.Gigascience. 2012 Nov 15;1(1):16. doi: 10.1186/2047-217X-1-16.

32. Li Z, Song C, Ouyang H, Lai L, Payne KJ, Dovat S. Cell cycle-specific function of Ikaros in human leukemia.Pediatr Blood Cancer. 2012 Jul 15;59(1):69-76. doi: 10.1002/pbc.23406. Epub 2011 Nov 21.

33. Wu X, Ouyang H, Duan B, Pang D, Zhang L, Yuan T, Xue L, Ni D, Cheng L, Dong S, Wei Z, Li L, Yu M, Sun QY, Chen DY, Lai *L, Dai Y*, Li GP*. Production of cloned transgenic cow expressing omega-3 fatty acids Transgenic Res. 2011 Sep 15. [Epub ahead of print].

34. Wei J, Ouyang H, Wang Y, Pang D, Cong NX, Wang T, Leng B, Li D, Li X, Wu R, Ding Y, Gao F, Deng Y, Liu B, Li Z, Lai L, Feng H, Liu G, Deng XCharacterization of A Hypertriglyceridemic Transgenic Miniature swine Model expressing Human Apolipoprotein CIII. FEBS J. 2011 Oct 24. doi: 10.1111/j.1742-4658.2011.08401.x. [Epub ahead of print].

35. Tian J, Song J, Li H, Yang D, Li X, Ouyang H, Lai L.Effect of Donor Cell Type on Nuclear Remodelling in Rabbit Somatic Cell Nuclear Transfer Embryos. Reprod Domest Anim. 2011 Oct 29. doi: 10.1111/j.1439-0531.2011.01915.x. [Epub ahead of print].

36. Huang Y, Tang X, Xie W, Zhou Y, Li D, Yao C, Zhou Y, Zhu J, Lai L, Ouyang H, Pang D. Histone Deacetylase Inhibitor Significantly Improved the Cloning Efficiency of Porcine Somatic Cell Nuclear Transfer Embryos.Cell Reprogram. 2011 Oct 26. [Epub ahead of print].

37. Huang Y, Tang X, Xie W, Zhou Y, Li D, Zhou Y, Zhu J, Yuan T, Lai L, Pang D, Ouyang H.Vitamin C enhances in vitro and in vivo development of porcine somatic cell nuclear transfer embryos. Biochem Biophys Res Commun. 2011 Jul 29;411(2):397-401. Epub 2011 Jul 2.

38. Wei D., Yang D., Zhao B., Ouyang Z., Song J.,  Fan N., Liu L., Zhao Z.， Wu Q., Nashun B., Tang J.，Wu Z., Gu W*, Lai L*. Use of the 2A Peptide for Generation of Multi-transgenic Pigs through a Single Round of Nuclear Transfer. PLoS One. 2011;6(5):e19986. Epub 2011 May 13

39. Huang Y, Tang X, Xie W, Zhou Y, Li D, Zhou Y, Zhu J, Yuan T, Lai L, Pang D, OuyangH.Vitamin C enhances in vitro and in vivo development of porcine somatic cell nuclear transfer embryos BiochemBiophys Res Commun. 2011 Jul 29;411(2):397-401. Epub 2011 Jul 2

40. Yang D., Yang H., Li W., Zhao B., Ouyang Z., Liu Z., Zhao Y., Fan N., Song J., Tian J,. Li F., Zhang J., Chang L., Pei D., Chen Y.E. *, Lai L*. Generation of PPARγ mono-allelic knockout pigs via zinc-finger nucleases and nuclear transfer cloning. Cell Res. 2011 Jun;21(6):979-82. Epub 2011 Apr 19.

41. Huang L, Fan N, Cai J, Yang D, Zhao B, Ouyang Z, Gu W*, Lai *L. Establishment of a Porcine Oct-4 Promoter-Driven EGFP Reporter System for Monitoring Pluripotency of Porcine Stem Cells Cell Reprogram. 2011 Apr;13(2):93-8. Epub 2011 Jan 23.

42. Park J, Lai L, Samuel M, Wax D, Bruno RS, French R, Prather RS, Yang X, Tian XC. Altered Gene Expression Profiles in the Brain, Kidney, and Lung of One-Month-Old Cloned Pigs. Cell Reprogram. 2011 Mar 31. [Epub ahead of print].

43. Liao B, Bao X, Liu L, Feng S, Zovoilis A, Liu W, Xue Y, Cai J, Guo X, Qin B, Zhang R, Wu J, Lai L, Teng M, Niu L, Zhang B, Esteban MA, Pei D. J Biol Chem.Microrna cluster 302-367 enhances somatic cell reprogramming by accelerating a mesenchymal-to-epithelial transition 2011 Mar 29. [Epub ahead of print].

44. Chen J, Liu J, Han Q, Qin D, Xu J, Chen Y, Yang J, Song H, Yang D, Peng M, He W, Li R, Wang H, Gan Y, Ding K, Zeng L, Lai L, Esteban MA, Pei D. Towards an optimized culture medium for the generation of mouse induced pluripotent stem cells. J Biol Chem. 2010 Oct 1;285(40):31066-72. Epub 2010 Jul 1.

45. Yang D, Wang CE, Zhao B, Li W, Ouyang Z, Liu Z, Yang H, Fan P, O'Neill A, Gu W, Yi H, Li S, Lai L*, Li XJ*.Expression of Huntington's disease protein results in apoptotic neurons in the brains of cloned transgenic pigs. Hum Mol Genet. 2010 19:3983-3994

46. .Yang J, Cai J, Zhang Y, Wang X, Li W, Xu J, Li F, Guo X, Deng K, Zhong M, Chen Y, Lai L, Pei D, Esteban MA. Induced pluripotent stem cells can be used to model the genomic imprinting disorder Prader-Willi syndrome J Biol Chem. 2010 Dec 17;285(51):40303-11. Epub 2010 Oct 18.

47. Li R, Liang J, Ni S, Zhou T, Qing X, Li H, He W, Chen J, Li F, Zhuang Q, Qin B, Xu J, Li W, Yang J, Gan Y, Qin D, Feng S, Song H, Yang D, Zhang B, Zeng L, Lai L, Esteban MA, Pei D. A mesenchymal-to-epithelial transition initiates and is required for the nuclear reprogramming of mouse fibroblasts. Cell Stem Cell. 2010 Jul 2;7(1):51-63. Epub 2010 Jun 17

48. Park J, Marjani SL, Lai L, Samuel M, Wax D, Davis SR, Bruno RS, Prather RS, Yang X, Tian XC. Altered gene expression profiles in the brain, kidney, and lung of deceased neonatal cloned pigs.Cell Reprogram. 2010 Oct;12(5):589-97

49. Chen J, Liu J, Han Q, Qin D, Xu J, Chen Y, Yang J, Song H, Yang D, Peng M, He W, Li R, Wang H, Gan Y, Ding K, Zeng L, Lai L, Esteban MA, Pei D.Towards an optimized culture medium for the generation of mouse induced pluripotent stem cells. J Biol Chem. 2010 Oct 1;285(40):31066-72. Epub 2010 Jul 1

50. Esteban MA, Xu J, Yang J, Peng M, Qin D, Li W, Jiang Z, Chen J, Deng K, Zhong M, Cai J, Lai L, Pei D J. Biol Chem. Generation of induced pluripotent stem cell lines from Tibetan miniature pig . 2009, 284:17634-17640

51. Li L, Pang D, Wang T, Li Z, Chen L, Zhang M, Song N, Nie D, Chen Z, Lai L, Ouyang H.Biochem Biophys Res Commun. Production of a reporter transgenic pig for monitoring Crerecombinase activity. 2009.382:232-235.

52. Esteban M.A., Peng M., Deli Z., Cai J., Yang J., Xu J., Lai .L, Pei D. IUBMB Life. Porcine induced pluripotent stem cells may bridge the gap between mouse and human iPS. IUBMB Life. 2010, 62:277-282.

53. Isom SC, Lai L, Prather RS, Rucker EB 3rd.  Heat shock of porcine zygotes immediately after oocyte activation increases viability. MolReprod Dev. 2008 Oct 24. [Epub ahead of print]

54. Lu Y, Nie D, Witt WT, Chen Q, Shen M, Xie H, Lai L, Dai Y, Zhang J.  Expression of the fat-1 gene diminishes prostate cancer growth in vivo through enhancing apoptosis and inhibiting GSK-3{beta} phosphorylation. Mol Cancer Ther. 2008 Oct;7(10):3203-11.

55. Bonk AJ, Li R, Lai L, Hao Y, Liu Z, Samuel M, Fergason EA, Whitworth KM, Murphy CN, Antoniou E, Prather Aberrant DNA methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transfer-produced blastocysts. Mol Reprod Dev. 2008 Feb;75(2):250-64.

56. Lu Y, Nie D, Witt WT, Chen Q, Shen M, Xie H, Lai L, Dai Y, Zhang J. Expression of the fat-1 gene diminishes prostate cancer growth in vivo through enhancing apoptosis and inhibiting GSK-3 beta phosphorylation.Mol Cancer Ther. 2008 Oct;7(10):3203-11.

57. Bonk AJ, Cheong HT, Li R, Lai L, Hao Y, Liu Z, Samuel M, Fergason EA, Whitworth KM, Murphy CN, Antoniou E, Prather RS. Correlation of developmental differences of nuclear transfer embryos cells to the methylation profiles of nuclear transfer donor cells in Swine. Epigenetics. 2007 Sep;2(3):179-86

58. Zhong Z, Spate L, Hao Y, Li R, Lai L, Katayama M, Sun QY, Prather RS, Schatten H.Remodeling of Centrosomes in Intraspecies and Interspecies Nuclear Transfer Porcine Embryos. Cell Cycle. 2007 Apr 16;6(12).

59. Jiang L, Lai L, Samuel M, Prather RS, Yang X, Tian XCExpression of X-linked genes in deceased neonates and surviving cloned female piglets. MolReprod Dev. 2008 Feb;75(2):265-73.

60. Jiang L, Jobst P, Lai L, Samuel M, Ayares D, Prather RS, Tian XC Expression levels of growth-regulating imprinted genes in cloned piglets. Cloning Stem Cells.2007 9(1):97-106.

61. Im GS, Samuel M, Lai L, Hao Y, Prather RS. Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion. Mol Reprod Dev. 2007 Mar 15; [Epub ahead of print

62. Li R, Whitworth K, Lai L, Wax D, Spate L, Murphy CN, Rieke A, Isom C, Hao Y, Zhong Z, Katayama M, Schatten H, Prather RS.Concentration and composition of free amino acids and osmolalities of porcine oviductal and uterine fluid and their effects on development of porcine IVF embryos. Mol Reprod Dev. 2007 Sep;74(9):1228-35.

63. Martin L, Besch-Williford C, Lai L, Cheong HT, Im GS, Park KW, Murphy C, Hao Y, Ellersieck MR, Keisler DH, Schatten H, Green JA, Prather RS. Morphologic and histologic comparisons between in vivo and nuclear transfer derived porcine embryos. Mol Reprod Dev. 2007 Aug;74(8):952-60.

64. Generation of cloned transgenic pigs rich in omega-3 fatty acids. Lai L, Kang JX, Li R, Wang J, Witt WT, Yong HY, Hao Y, Wax DM, Murphy CN, Rieke A, Samuel M, Linville ML, Korte SW, Evans RW, Starzl TE, Prather RS, Dai Y.NatBiotechnol. 2006 Apr;24(4):435-6. Epub 2006 Mar 26.

65. Hao YH, Yong HY, Murphy CN, Wax D, Samuel M, Rieke A, Lai L, Liu Z, Durtschi DC, Welbern VR, Price EM, McAllister RM, Turk JR, Laughlin MH, Prather RS, Rucker EB.Production of endothelial nitric oxide synthase (eNOS) over-expressing piglets. Transgenic Res. 2006 Dec;15(6):739-50.

66. Bjerregaard B, Pedersen HG, Jakobsen AS, Rickords LF, Lai L, Cheong HT, Samuel M, Prather RS, Strejcek F, Rasmussen ZR, Laurincik J, Niemann H, Maddox-Hyttel P, Thomsen PD.Activation of ribosomal RNA genes in porcine embryos produced in vitro or by somatic cell nuclear transfer. Mol Reprod Dev. 2007 Jan;74(1):35-41.

67. Mika Katayama, ZhishengZhong, Liangxue Lai , Peter Sutovsky, Randall S. Prather and HeideSchatten Mitochondrial distribution and microtubule organization in fertilized and cloned porcine embryos: Implications for developmental potential. Dev Biol. 2006 Nov 1;299(1):206-20.

68. Rongfeng Li, Liangxue Lai(Equal contribution with first author), David Wax, YanhongHao, Clifton Murphy, August Rieke, Melissa Samuel, Mike Linville, Scott Korte, Rhobert W. Evans, Yifan Dai, Randall S. Prather Cloned Transgenic Swine via In Vitro Production and Cryopreservation, Biol Reprod, 2006 75(2):226-30.

69. Hao YH, Lai LX, Liu ZH, Im GS, Wax D, Samuel M, Murphy CN, Sutovsky P, Prather RS.Developmental competence of porcine parthenogenetic embryos relative to embryonic chromosomal abnormalities. Mol Reprod Dev. 2006 Jan;73(1):77-82.

70. Vajta G, Maddox-Hyttel P, Skou CT, Tecirlioglu RT, Peura TT, Lai L, Murphy CN, Prather RS, Kragh PM, Callesen H.Highly efficient and reliable chemically assisted enucleation method for handmade cloning in cattle. Reprod Fertil Dev. 2005;17(8):791-7.

71. Liu Z, Schatten H, Hao Y, Lai L, Wax D, Samuel M, Zhong ZS, Sun QY, Prather RS.The Nuclear Mitotic Apparatus (NuMA) protein is contributed by the donor cell nucleus in cloned porcine embryos. : Front Biosci. 2006 May 1;11:1945-57

72. Yong HY, Hao Y, Lai L, Li R, Murphy CN, Rieke A, Wax D, Samuel M, Prather RS. Production of a transgenic piglet by a sperm injection technique in which no chemical or physical treatments were used for oocytes or sperm. Mol Reprod Dev. 2006 May;73(5):595-9

73. Manandhar G, Feng D, Yi YJ, Lai L, Letko J, Laurincik J, Sutovsky M, Salisbury JL, Prather RS, Schatten H, Sutovsky PCentrosomal protein centrin is not detectable during early pre-implantation development but reappears during late blastocyst stage in porcine embryos. Reproduction. 2006 Sep;132(3):423-34.

74. Hao Y, Mathialagan N, Walters E, Mao J, Lai L, Becker D, Li W, Critser J, PratherOsteopontin reduces polyspermy during in vitro fertilization of porcine oocytes. Biol Reprod. 2006 Nov;75(5):726-33. Epub 2006 Jul 26

75. Sutovsky P, Manandhar G, Laurincik J, Letko J, Caamano JN, Day BN, Lai L, Prather RS, Sharpe-Timms KL, Zimmer R, Sutovsky M Expression and proteasomal degradation of the major vault protein (MVP) in mammalian oocytes and zygotes.Reproduction. 2005 Mar;129(3):269-82

76. Im GS, Yang BS, Lai L, Liu Z, Hao Y, Prather RS. Fragmentation and development of preimplantation porcine embryos derived by parthenogenetic activation and nuclear transfer.MolReprod Dev. 2005 Jun;71(2):159-65.

77. Donna Kolber-Simonds, Liangxue Lai (Equal contribution with first author), Steven R. Watt, Maria Denaro, Scott Arn, Monica L. Augenstein, Jeffery Betthauser, David B. Carter, Julia L. Greenstein, YanhongHao, Gi-Sun Im, Zhonghua Liu, Greg D. Mell, Clifton N. Murphy, Kwang-Wook Park, August Rieke, David Ryan, David H. Sachs, Erik J. Forsberg, Randall S. Prather and Robert J. Hawley. α -1,3-galactosyltransferase null pigs via nuclear transfer with fibroblasts bearing loss of heterozygosity mutations. ProcNatlAcadSci U S A. 2004 11;101(19):7335-40.

78. Wu GM, Lai L, Mao J, McCauley TC, Caamano JN, Cantley T, Rieke A, Murphy CN, Prather RS, Didion BA, Day BN.Birth of piglets by in vitro fertilization of zona-free porcine oocytes. Theriogenology. 2004 Nov;62(8):1544-56.

79. Gi-Sun Im, Liangxue Lai, Zhonghua Liu, YanhongHao, David Wax, Aaron Bonk and Randall S. Prather. In vitro development of preimplantation porcine nuclear transfer embryos cultured in different media and gas atmospheres.Theriogeneology  2004 5-1135.

80. Liangxue Lai and Randall S Prather. Cloning Pigs as Donors for Organ Transfers in Human, Promises and Challenges. IEEE Eng Med Biol Mag. 2004 23(2):37-42

81. Thompson WE, Asselin E, Branch A, Stiles JK, Sutovsky P, Lai L, Im GS, Prather RS, Isom SC, Rucker E 3rd, Tsang BK.Regulation of prohibitin expression during follicular development and atresia in the mammalian ovary. BiolReprod. 2004 Jul;71(1):282-90. 2004.

82. Hao Y, Lai L, Mao J, Im GS, Bonk A, Prather RS. Apoptosis in parthenogeneticpreimplantation porcine embryos. BiolReprod. 2004 Jun;70(6):1644-9. 2004

83. Liangxue Lai, Randall S. Prather. Creating genetically modified pigs by using nuclear transfer, Reproductive Biology and Endocrinology, 2003, 1:82

84. Liangxue Lai, Randall S Prather. Production of cloned pigs by using somatic cells as donors. Cloning and Stem cells, 2003,  5:233-241

85. YanhongHao, Liangxue Lai, Jiude Mao, Gi-Sun Im, Aaron Bonk, Randall S. Prather. Apoptosis and in vitro development of preimplantation porcine embryos derived in vitro or by nuclear transfer. BiolReprod. 2003 69:501-7.

86. Randall S. Prather , Rober J. Hawley, David B.Carter, Liangxue Lai, Julie Greenstein. Transgenic swine for biomedicine and agriculture. Theriogenology. 2003 59:115-23.

87. Lai L, Kolber-Simonds D, Park KW, Cheong HT, Greenstein JL, Im GS, Samuel M, Bonk A, Rieke A, Day BN, Murphy CN, Carter DB, Hawley RJ, Prather RS. Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning.Science. 2002 Feb 8;295(5557):1089-92. Epub 2002 Jan 3

88. Liangxue Lai, Kwang-Wook Park, Hee-Tae Cheong, Birgit Kühholzer, Melissa Samuel, Aaron Bonk, Gi-Sun Im, August Rieke, Billy N. Day, Clifton N. Murphy, David B. Carter, Randall S. Prather. A transgenic pig expressing the enhanced green fluorescent protein produced by nuclear transfer using colchicines-treated fibroblasts as donor cells. Molecular Reproduction and Development, 2002 62:300-306.

89. Hee-Tae Cheong, Kwang-Wook Park, Gi-Sun Im, Liangxue Lai, Qing-Yuan Sun, Billy N. Day, Randall S. Prather. Effect of elevated Ca+2 concentration in fusion/activation medium on the fusion and development of porcine fetal fibroblast nuclear transfer embryos. Molecular Reproduction and Development. 2002 61:488-92.

90. Guang-Ming Wu, Qing-Yuan Sun, Jiude Mao, Liangxue Lai, Tod C. McCauley, Kwang-Wook Park, Randall S. Prather, Brad A. Didion and Billy N. Day. High Developmental Competence of Pig Oocytes after Meiotic Inhibition with a Specific M-Phase Promoting Factor Kinase Inhibitor, Butyrolactone I, Biology of Reproduction, 2002 67:170-177.

91. Bart D.  Carter, Liangxue Lai, Kwang-Wook Park, Melissa Samuel, Jimmy C. Lattimer, D. Mark Estes, Cynthia Besch-Williford, and Randall S. Prather. Phenotyping of Transgenic Cloned Piglets. Cloning and Stem cell. 2002 4:131-145

92. Liangxue Lai, Randall S. Prather. Progress in Producing Knockout models for Xenotransplantation by Nuclear Transfer. Annals of Medicine. 2002, 34:1-6.

93. Qing-Yuan Sun, Guangming Wu, Liangxue Lai, Aaron Bonk , Ryan Cabot, Kwang-Wook Park, Bill N Day, Randall S Prather, Heidi Schatten. Regulation of Mitogen-Activated Protein Kinase Phosphorylation, Microtubule Organization, Chromatin Behavior, and Cell Cycle Progression by Protein Phosphatases During Pig Oocyte Maturation and Fertilization In Vitro. Biology of Reproduction 2002 66:580-588.

94. Liangxue Lai, Qing-Yuan Sun, GuangmingWu, Clifton N Murphy, Birgit Kuhholzer, Kwang-Wook Park, Aaron Bonk, Bill N Day, Randall S Prather. Development of porcine embryos and offspring after intracytoplasmic sperm injection with liposome transfected or non-transfected sperm into in vitro matured oocytes. Zygote. 2001 9:339-46.

95. Liangxue Lai, Tao Tao, ZoltanMachaty, Birgit Kuhholzer, Qing-Yuan Sun, Kwang-Wook Park, Bill N Day, Randall S Prather. Feasibility of producing porcine nuclear transfer embryos by using G2/M-stage fetal fibroblasts as donors. Biology of Reproduction. 2001 65:1558-64.

96. Ryan A Cabot, Birgit Kuhholzer, Anthony W Chan, Liangxue Lai, Kwang-wook Park, Chong KY, Schatten G, Murphy CN, Abeydeera LR, Bill N Day, Randall S Prather RS. Transgenic pigs produced using in vitro matured oocytes infected with a retroviral vector. Animal Biotechnology 2001 12:205-14.

97. Kang-Wook Park, Hee-Tae Cheong, Liangxue Lai, Gi-Sun Im, Birgit Kuhholzer, Aaron Bonk, Melissa Samuel, August Rieke, Bill N. Day, Clifton N. Murphy, David B.Carter, Randall S Prather. Production of nuclear transfer-derived swine that express the enhanced green fluorescent protein. Animal Biotechnology, 2001 12:173-81

98. Qing-Yuan Sun, Liangxue Lai, Guangming Wu, Kang-Wook Park, Bill N Day, Randall S Prather, Heidi Schatten. Microtubule assembly after treatment of pig oocytes with taxol: correlation with chromosomes, gamma-tubulin, and MAP kinase. Molecular Reproduction and Development. 2001 60:481-90.

99. Kwang-Wook Park, Liangxue Lai, Hee-Tae Cheong, Ryan Cabot, Qing-Yuan Sun, GuangmingWu, Bill N Day, Randall S Prather. Mosaic gene expression in nuclear transfer-derived embryos and the production of cloned transgenic pigs from ear-derived fibroblasts. Biology of Reproduction, 2002; 66:1001-5.

100. Kwang-Wook Park, Liangxue Lai, Hee-Tae Cheong, Gi-Sun Im, Qing-Yuan Sun, GuangmingWu, Bill N Day, Randall S Prather. Developmental potential of porcine nuclear transfer embryos derived from transgenic fetal fibroblasts infected with the gene for the green fluorescent protein: comparison of different fusion/activation conditions. Biology of Reproduction, 2001 65:1681-5.

101. Kwang-Wook Park, Birgit Kuhholzer, Liangxue Lai, ZoltanMachaty, Qing-Yuan Sun, Bill N Day, Randall S Prather RS. Development and expression of the green fluorescent protein in porcine embryos derived from nuclear transfer of transgenic granulosa-derived cells. Animal Reproduction Science. 2001 68:111-20.

102. Qing-Yuan Sun, Liangxue Lai, Randall S Prather, Heidi Schatten. Antioxidants stimulate meiosis resumption, but inhibit mitogen-activated protein kinase phosphorylation and further cell cycle progression in porcine oocytes. Reproduction and Fertility. 2000;12:383-9.

103. Qing-Yuan Sun, GuangmingWu, Liangxue Lai, Kwang-Wook Park, Ryan Cabot, Hee-Tae Cheong, Bill N Day, Randall S Prather RS, Heidi Schatten. Translocation of active mitochondria during pig oocyte maturation, fertilization and early embryo development in vitro. Reproduction. 2001 12:155-63.

104. Birgit Kuhholzer, Robert J Hawley, Liangxue Lai, Donna Kolber-Simonds, Randall S Prather. Clonal lines of transgenic fibroblast cells derived from the same fetus result in different development when used for nuclear transfer in pigs. Biology of Reproduction, 2001 64:1695-8.

105. Qing-Yuan Sun, Liangxue Lai, Kwang-wook Park, BirrgitKuhhozer, Randall S Prather, Heidi Schattern. Dynamic events are differently mediated by microfilaments, microtubules, and mitogen-activated protein kinase during porcine oocyte maturation and fertilization in vitro. Biology of Reprodoction, 2001, 64: 879-889.

106. Qing-Yuan Sun, Liangxue Lai, Aaron Bonk, Randall S Prather, Heidi Schatten. Cytoplasmic changes in relation to nuclear maturation and early embryo developmental potential of porcine oocytes: effects of gonadotropins, cumulus cells, follicular size, and protein synthesis inhibition. Molecular Reproduction and Development, 2001 59:192-8.

107. Randall S Prather, Birgit Kuhholzer, Liangxue Lai, Kwang-Wook Park. Changes in structure of nuclei after transfer to oocytes. Cloning, 2000, 2: 117-121.

     Chapter book

     Methods in Molecular medicine, “A Method for Producing Cloned pigs by Using Somatic Cells as Donors”, Liangxue Lai, Randall S Prather, 2003, , Published by Human Press Co, USA.

     Encyclopedia of Animal Science, “Animal cloning”.Liangxue Lai, Randall S Prather, 2005.

     Comparative Reproductive Biology, “Animal Cloning” , Liangxue Lai, Randall S Prather, Published by Blackwell Publishing USA, 2007